1) Detection of CD3-CD56+CD16-/+
Human Blood Lymphocytes by Flow Cytometry.
Cell: Human peripheral blood lymphocytes

Stained: (1) PE-conjugated Mouse Anti-Human NCAM-1/CD56 MAb (Cat# FAB2408P)
(2) Fluorescein-conjugated Mouse Anti-Human Fcγ RIIIA/B/CD16 MAb (Cat#
FAB2546F)

Gated: CD3- lymphocytes

2) NKp46 on Human Peripheral Blood Lymphocytes

Cell: Human peripheral blood lymphocytes

Stained: (1) PE-conjugated Mouse Anti-Human NCAM-1/CD56 MAb (Cat# FAB2408P),
(2)Fluorescein-conjugated Mouse Anti-Human NKp46 MAb (Cat#
FAB1850F)

 

3) B7-H6 on HeLa Cells

Cell: HeLa human cervical epithelial carcinoma cell

Stained: (1) APC-conjugated Mouse Anti-Human B7-H6 MAb (Cat# FAB7144A) or
(2) APC-conjugated Mouse IgG
1 Isotype Control (Cat#
IC002A)

 

CD3 CD56 Fcγ RIIA/B (CD16a/b) IFN-γ CD27/TNFRSF7
CD161/NK1.1 Integrin α2/CD49b Integrin αM/CD11b NKp46/NCR1 B7-H6
NKp30 NKp44 NKp80/KLRF1 CD94 NKG2A
NKG2C NKG2D H60 MICA MICB
MICA/B Rae-1 Pan Rae-1α/β/γ Rae-1ε ULBP-1
ULBP-2/5/6 ULBP-3 ULBP-4/RAET1E CD96 CD96v2
CRTAM DNAM-1/CD226 TIGIT CD155/PVR IGSF4A/SynCAM1/Necl2
Nectin-1 Nectin-2/CD112 Nectin-3/CD113 KIR pan/CD158 KIR2DL1/S5
KIR2DL3 KIR2DL4 KIR3DL1 KIR3DL2 KIR2DS4

 

Previous
Episode 2
Treg

NK Cell의 더 많은 내용이 궁금하다면 클릭!

Next
Episode 4. Dendritic Cells

 

 

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