Ãß°¡ÀÚ·á (Ŭ¸¯Çغ¸¼¼¿ä!)
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2014.08.20 |
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Antibody Array for Apoptosis |
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Apoptosis´Â ´Ù¾çÇÏ°í º¹ÀâÇÑ signal cascade¸¦ ÅëÇØ ÀϾ°Ô µÇ¸ç ¹ß»ý, ¾Ï, ½Å°æ ÅðÇ༺ Áúȯ µî¿¡¼ ¸Å¿ì Áß¿äÇÑ ±âÀÛ Áß Çϳª·Î ¾Ë·ÁÁ® ÀÖ½À´Ï´Ù. ¿õºñ¸ÞµðÅØ¿¡¼´Â Apoptosis °ü·Ã ´Ü¹éÁúµéÀÇ ¹ßÇöÀ» Çѹø¿¡ È®ÀÎÇÒ ¼ö ÀÖ´Â antibody array, apoptosis signalÀÇ key moleculeÀÎ caspaseÀÇ activity assay, apoptosis °ü·Ã ´Ù¾çÇÑ assay kitµîÀ» Á¦°øÇÏ°í ÀÖ½À´Ï´Ù. |
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Proteome Profiler Antibody Array Kit |
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Proteome Profiler? |
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- ¼Ò·®ÀÇ sample·Îµµ ½ÇÇè °¡´É
- Apoptosis specific marker 35°³ analyt ¸¦ ÇÑ ÀåÀÇ membrane¿¡ ³Ö¾ú½À´Ï´Ù! : Intrinsic pathway marker¿Í extrinsic pathway marker¸¦ °ñ°í·ç È®ÀÎÇÒ ¼ö ÀÖÀ½ : Bad, Bax, Bcl-2, cIAP-1, cIAP-2, Cytochrome c, FADD, Fas µî : Caspase-3ÀÇ pro form°ú cleaved formÀ» µ¿½Ã¿¡ È®ÀÎ °¡´É! : p53ÀÇ phosphorylation siteº°·Î ¹ßÇö¾ç Â÷ÀÌ È®ÀÎ °¡´É (S15, S46, S392)
- Most referenced array! : Array »ç¿ë ³í¹® Áß 91%°¡ R&D Á¦Ç° »ç¿ë
- Western blot Àåºñ¸¸À¸·Îµµ OK!
- Western blotº¸´Ù °£ÆíÇÑ ½ÇÇè ¹æ¹ý : ÀÚ¼¼ÇÑ ½ÇÇè ¹æ¹ýÀº µ¿¿µ»óÀ» ÅëÇØ È®ÀÎÇØÁÖ¼¼¿ä!
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ÀÌ·± ½ÇÇè¿¡ »ç¿ëÇغ¸¼¼¿ä! |
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- Ç×¾ÏÁ¦, Ç×¾Ï ¹°Áú¿¡ ÀÇÇÑ cancer cell death ±âÀÛ È®ÀÎ
- Apoptosis signalingÀ» ÇÑ ´«¿¡ È®ÀÎ
- Cell death°¡ ¾î¶² °æ·Î¸¦ ÅëÇØ ÀÌ·ç¾îÁö´ÂÁö È®ÀÎ °¡´É (Intrinsic ¶Ç´Â extrinsic)
¸¹Àº ¿¬±¸ÀÚµéÀÌ Apoptosis array kitÀ» ¾î¶² ½ÇÇè¿¡ »ç¿ëÇß´ÂÁö Âü°í³í¹®À» È®ÀÎÇØÁÖ¼¼¿ä! |
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»ç¿ë °¡´ÉÇÑ Array Á¦Ç° | ||||||||||
ÃßõÁ¦Ç° 1) Human Apoptosis Array Kit (35 analytes, Cat# ARY009)
ÃßõÁ¦Ç° 2) Human Cell Stress Array Kit (26 analytes, Cat# ARY018) |
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°ü·Ã Á¦Ç° ¹× °ü·Ã ÀÚ·á |
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°ü·Ã Á¦Ç° | ||||||||||
1. Cancer ¿¬±¸ÀÇ ÇʼöÇ°! Phospho-array kit Phopho-RTK, Phospho-MAPK, Phospho-Kinase °ü·Ã markerµéÀ» ÇÑ ÀåÀÇ membrane¿¡¼ È®ÀÎÇغ¸¼¼¿ä! |
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2. TUNEL Assays DNA fragmentationÀº apoptotic cellÀÇ °¡Àå Å« Ư¡ Áß ÇϳªÀ̸ç TUNEL assayÀº DNA fragmenta¸¦ È®ÀÎÇÒ ¼ö ÀÖ´Â °¡Àå º¸ÆíÀûÀÎ ½ÇÇè ¹æ¹ýÀÔ´Ï´Ù. R&D systems¿¡¼´Â °í³óµµ·Î Á¤ÀçµÈ TdT enzymeÀ» Æ÷ÇÔÇÑ TUNEL assay kitÀ» Á¦°øÇÏ°í ÀÖÀ¸¸ç, ¼±»ý´Ô²²¼ ¿øÇϽô ½ÇÇè ¹æ¹ý¿¡ ²À ¸Â´Â ´Ù¾çÇÑ TUNEL assay kitÀ» Á¦°øÇÏ°í ÀÖ½À´Ï´Ù. (ÀüÀÚÇö¹Ì°æ ¹æ½Ä, Çü±¤/flow-cytometry ¹æ½Ä, microplate reader, Á¶Á÷ ƯÀÌÀû colorimetric) |
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3. ApoStat Apoptosis Detection Kit ApopStat´Â flow cytometry¹æ½ÄÀ» ÀÌ¿ëÇؼ apoptotic cell¿¡¼ÀÇ caspase È°¼º Á¤µµ¸¦ Á¤·®ÇÏ´Â Á¦Ç°ÀÔ´Ï´Ù. |
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R&D systems¿¡¼´Â biotin (for fixed cell), fluorescein (for unfixed cell) µÎ Á¾·ùÀÇ Annexin V Á¦Ç°ÀÌ Ãâ½ÃµÇ°í ÀÖ½À´Ï´Ù. |
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5. Mitochondrial Membrane Potential Disruption Assay MitochondriaÀÇ ¸·ÀüÀ§ ºØ±«´Â apoptosis Ãʱ⿡ ³ªÅ¸³ª´Â Çö»óÀÔ´Ï´Ù. TheDePsipher¢â assay kitÀº lipophilic actionÀ» ÀÌ¿ëÇؼ mitochondria membrane potential º¯È¸¦ È®ÀÎÇÒ ¼ö ÀÖ´Â Á¦Ç°ÀÔ´Ï´Ù. |
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R&D systems¿¡¼´Â ELISAÀÇ gold-standardÀÎ Quantikine ELISA kitÀ» ºñ·ÔÇÏ¿© caspase activity assay, ³ôÀº È¿À²À» ÀÚ¶ûÇÏ´Â protein°ú antibody ¹× ´Ù¾çÇÑ Á¾·ùÀÇ caspase inhibitorµéÀ» Á¦°øÇÏ°í ÀÖ½À´Ï´Ù. (Pro form, active form ¸ðµÎ ÀÌ¿ë °¡´É) |
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MTTº¸´Ù ¶Ù¾î³ °¨µµ, ¼¼Æ÷¿¡ ¿µÇâÀ» ÁÖÁö ¾Ê´Â ¹«µ¶¼º, suspension cell¿¡¼µµ ¿ì¼öÇÑ ÀçÇö¼ºÀ» º¸ÀÌ´Â CCK-8! |
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8. Additional Apoptosis-related Detection kits and Assays Apoptosis transcription factor, Bcl-2 family, caspase, Heat shock protein, IAP µî, apoptosis¿Í °ü·ÃµÈ ´Ù¾çÇÑ Á¦Ç°µéÀ» ¼Ò°³ÇØ µå¸³´Ï´Ù. |
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9. Small Molecules for Apoptosis Research Cell death¸¦ ÀÏÀ¸Å² ƯÁ¤ pathway¸¦ Á¤ÀÇÇÒ »Ó¸¸ ¾Æ´Ï¶ó apoptosis¸¦ ÀÏÀ¸Å² factor¸¦ Á¤ÀÇÇϱâ À§Çؼ´Â small moleculeÀ» ÀÌ¿ëÇÑ ½ÇÇèÀÌ Áß¿äÇÕ´Ï´Ù. R&D systemsÀÇ ÀÚȸ»çÀÎ TOCRIS¿¡¼ Á¦°øÇÏ´Â apoptosis °ü·Ã small moleculeµéÀ» ¸¸³ªº¸¼¼¿ä! |
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´Ù¾çÇÑ ÁúȯÀÇ ¿øÀÎÀÌ µÇ´Â oxidative stress markerµµ R&D systems ELISA·Î Á¤È®ÇÏ°í °£ÆíÇÏ°Ô ÃøÁ¤Çϼ¼¿ä! |
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°ü·Ã ÀÚ·á | ||||||||||
ÀüÈ ¶Ç´Â ¸ÞÀÏ·Î ¿¬¶ô Áֽøé ÇÊ¿äÇÑ ÀڷḦ Àü´ÞÇØ µå¸®µµ·Ï ÇÏ°Ú½À´Ï´Ù. |
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Á¦Ç° »ó´ã : Çмú¸¶ÄÉÆÃÆÀ ÇѼҿµ (techserv@woongbee.com/ 031-776-3300 ³»¼±122) |
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