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2014. 03.26
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T Cell Enrichment Columns
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Negative T cell Isolation without Magnet
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Magnet? No! Cell loss? No! Cell Aggregation? No!
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High yield, High purity? Yes!
Why?? R&D Systems
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T Cell isolationÀº Çؾ߰ڴµ¥... MagnetÀÌ ¾ø¾î¼ °ÆÁ¤ÇÏ°í °è½Ê´Ï±î??
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¿Ö °ÆÁ¤ÇÏ°í °è½Ê´Ï±î?? T Cell Enrichment ColumnÀÌ Àִµ¥~~
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MagnetÀÌ ÇÊ¿ä¾ø°í CellÀ» ³Ö¾îÁֱ⸸ ÇÏ¸é µÇ¼ ºñ¿ë°ú ½Ã°£Àº ÁÙ¿©ÁÖ°í,
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°á°úÀÇ Ç°ÁúÀº ³ô¿©ÁÖ´Â T Cell Enrichment ColumnÀ¸·Î »ç¿ëÇغ¸¼¼¿ä!
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T Cell Enrichment Column ÀÇ Æ¯Â¡
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- 85-95%ÀÇ ³ôÀº Purity- ¼ø¼öÇÏ°Ô ¿øÇÏ´Â ¼¼Æ÷¸¸À» ³ôÀº ºñÀ²·Î ºÐ¸®ÇÒ ¼ö ÀÖ½À´Ï´Ù
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- Negative isolation- ¿øÇÏ´Â ¼¼Æ÷¸¦ ¼ø¼öÇÏ°Ô ºÐ¸®½ÃÄÑ Áֱ⠶§¹®¿¡ downstream¿¡ Á¦¾àÀÌ ¾ø½À´Ï´Ù
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- ºñ¿ë°ú ½Ã°£ Àý¾à- Ãß°¡ ±¸¼ºÇ° ¾øÀÌ ColumnÇϳª·Î ¸ðµç ½ÇÇèÀÌ °¡´ÉÇÏ°í, 30ºÐ À̳»¿¡ ½ÇÇèÀ» ³¡³¾ ¼ö ÀÖ½À´Ï´Ù
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- °£´ÜÇÑ ½ÇÇè ¹æ¹ý- column¿¡ antibody¿Í ¹ÝÀÀ½ÃŲ ¼¼Æ÷¸¦ Èê·ÁÁֱ⸸ ÇÏ¸é µÇ¼ ½ÇÇè¹æ¹ýÀÌ ¾ÆÁÖ °£´ÜÇÕ´Ï´Ù.
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- Human, Mouse, Rat type- °¢ ¼¼Æ÷ÀÇ Á¾¿¡ ÇØ´çµÇ´Â Á¦Ç°À¸·Î ¼±ÅÃÇÏ¿© »ç¿ëÇÏ½Ã¸é µË´Ï´Ù.
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T Cell Enrichment Column ÀÇ ¿ø¸®
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STEP1)
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Kit ³»¿¡ µé¾îÀÖ´Â monoclonal antibodyÀÇ cocktail°ú sampleÀ» ¹ÝÀÀ½Ãŵ´Ï´Ù.
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| ÀÏÁ¤ ½Ã°£ ¹ÝÀÀÀ» ½ÃÅ°¸é ºÐ¸®¸¦ ¿øÇÏ´Â subtype ¿ÜÀÇ ¼¼Æ÷µé°ú cocktail antibody °¡ °áÇÕÇÏ°Ô µË´Ï´Ù. |
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STEP2)
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Antibody¿Í ¹ÝÀÀ½ÃŲ ¼¼Æ÷µéÀ» column¿¡ ³Ö¾î Á߷¿¡ ÀÇÇØ ¾Æ·¡·Î È帣¸é¼ ºÐ¸®µË´Ï´Ù.
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Column¿¡´Â Ig°ú anti-Ig glass beads°¡ Æ÷ÇԵǾî ÀÖ¾î B cell°ú Monocyte¸¦ °É·¯ÁÝ´Ï´Ù.
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- Monocyte (Ig glass beads + Fc )
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- B cells (Anti-Ig glass bead + F(ab) surface Ig)
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Á¦Ç°±º
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¡Ú Á¦Ç° ¸®½ºÆ® º¸·¯°¡±â  |
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Human
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Mouse
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Rat
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CD3 T cells
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CD3+
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CD3+
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CD3+
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CD4 T cells
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CD4+
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CD4+
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CD4+
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CD8 T cells
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CD8+
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CD8+
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CD8+
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Naive cells
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CD4+ 45RO-
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CD4+ CD62L+ CD44 low
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CD8+ 45RO-
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Memory cells
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CD4+ 45RA-
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CD4+ CD62L- /CD44 high
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CD8+ 45RA-
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Supplemental Cell Selection Products
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Products
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Size
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Catalog
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Column Rack, 5 small holds
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1 Each
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RACK
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Column Rack, 3 Large holds
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1 Each
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LRACK
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Human Erythrocyte Lysing Kit
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1 Kit
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WL1000
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Mouse Erythrocyte Lysing Kit
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1 Kit
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WL2000
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Other Related Products
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Products
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Size
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Catalog
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Application
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MagCellect Human CD3+ T Cell Isolation Kit
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1 Kit
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MAGH101
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Cell isolation
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MagCellect Mouse CD3+ T Cell Isolation Kit
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1 Kit
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MAGH201
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Cell isolation
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MagCellect Rat CD3+ T Cell Isolation Kit
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1 Kit
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MAGR301
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Cell isolation
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Dynabeads® Human T-Expander CD3/CD28
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2 mL
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11452D
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Cell expansion
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CellXVivo Human Th1 Cell Differentiation Kit
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1 Kit
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CDK001
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Cell differentiation
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Human CD3 epsilon PE MAb
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100 Test
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FAB100P
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Flow Cytometry
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Mouse CD3 PE MAb
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100 Test
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FAB4841P
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Flow Cytometry
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Related Information
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¡Ú T cell Enrichment ColumnÀ» »ç¿ëÇÏ½Ã¸é¼ ¹ß»ýÇÏ´Â ÀüÇüÀûÀÎ ¹®Á¦µé¿¡ ´ëÇÑ ÇØ°áÃ¥ 
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¡Ú Embryonic Stem Cell·ÎºÎÅÍ T cellÀ» ¸¸µå´Â ½¬¿î ¹æ¹ý 
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¡Ú T cell subtype Æ÷½ºÅÍ ¹«·á·Î ¹Þ±â 
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Á¦Ç° »ó´ã : Çмú¸¶ÄÉÆÃÆÀ Ãֹ̼± (techserv@woongbee.com/ 031-776-3300 ³»¼±123)
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