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2014. 06. 11.
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CCK-8 vs MTT ºñ±³ µ¿¿µ»ó
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CCK-8 ¸¸ÀÇ Æ¯ÀåÁ¡
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- MTT, XTT, MTS, WST-1 ´ëºñ 2~3¹è ¶Ù¾î³ °¨µµ
- 24½Ã°£ incubation¿¡µµ ¼¼Æ÷°¡ Á×Áö ¾Ê´Â ¹«µ¶¼º
- ³ÃÀå°í¿¡¼ ²¨³» ¹Ù·Î ¾²´Â Æí¸®¼º
- Long Stability (³ÃÀå 1³â)
- Suspension Cell ¿¡¼µµ ¿ì¼öÇÑ ÀçÇö¼º
- ºû¿¡ ÀÇÇÑ º¯¼ºÀÌ °ÅÀÇ ¾ø´Â ¾ÈÁ¤¼º
- ¹æ»ç¼º µ¿À§¿ø¼Ò, DMSO ºÒÇÊ¿ä
- Harvest, Washing, Solubilization °úÁ¤ ºÒÇÊ¿ä.
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³ÃÀå°í¿¡¼ ²¨³»¼ ¹Ù·Î ³Ö°í ÂïÀ¸¸é ³¡!!! CCK-8Àº preparation
°úÁ¤°ú dissolve °úÁ¤ÀÌ ÇÊ¿ä ¾ø½À´Ï´Ù. Æí¸®ÇÔµµ ÀåÁ¡ÀÌÁö¸¸
Suspersion cell¿¡µµ Àû¿ë °¡´ÉÇÏ´Ù´Â Å« ÀåÁ¡ÀÌ ÀÖ½À´Ï´Ù.
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¼¼Æ÷¿Í ½ÇÇèÀÚ ¸ðµÎ¿¡°Ô ¾ÈÀüÇÑ Á¦Ç°!! MTS¿Í MTT´Â ¹ÝÀÀ ÈÄ
ºÒ°ú 3½Ã°£ ¸¸¿¡ ¼¼Æ÷µéÀÌ »ç¸êµÇÁö¸¸, CCK-8Àº ¹«·Á 24½Ã°£
¹ÝÀÀ¿¡µµ ¼¼Æ÷µéÀÌ °Ç°ÇÏ°Ô À¯ÁöµË´Ï´Ù.
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SensitivityÀÇ È®¿¬ÇÑ Â÷ÀÌ°¡ º¸À̽óª¿ä? MTT¿¡¼ ½ÇÇ豺°ú
´ëÁ¶±º »çÀÌ¿¡ Â÷ÀÌ°¡ º¸ÀÌÁö ¾Ê´Â´Ù¸é MTTÀÇ ³·Àº sensitivity
¶§¹®ÀÏ ¼ö ÀÖ½À´Ï´Ù. ¹«·á »ùÇ÷ΠÅ×½ºÆ® Çغ¸¼¼¿ä (¾Æ·¡ ÂüÁ¶)
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ÇÊ¿äÇÒ¶§ ¸¶´Ù ³ìÀÌ°í.. ¼¯°í.. ºÒÆíÇÏÁö ¾ÊÀ¸¼¼¿ä? ¶Ç, ³²Àº ½Ã¾à
ÀÇ È°¼ºÀÌ ¶³¾îÁöÁö´Â ¾Ê¾ÒÀ»Áö ºÒ¾ÈÇÏÁö ¾ÊÀ¸¼¼¿ä? CCk-8Àº
³ÃÀ庸°üÀ¸·Î 1³â µ¿¾ÈÀ̳ª È°¼ºÀÌ À¯ÁöµË´Ï´Ù.
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CCK-8 ¹«·á »ùÇà Á¦°ø¾È³»
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CCK-8À» ½áº¸°í´Â ½ÍÀºµ¥, Ȥ½Ã¶óµµ ½ÇÇè ȯ°æ¿¡ ¸ÂÁö ¾ÊÀ»±îºÁ ¼±¶æ ±¸ÀÔÀÌ ¸Á¼³¿©
Áö½ÃÁÒ? ÀúÈñ ¿õºñ ¸ÞµðÅØ¿¡¼´Â ¼±»ý´ÔµéÀÇ ±×·± ºÒ¾ÈÀ» ´ú¾îµå¸®°íÀÚ CCK-8 »ùÇÃÀ»
¹«·á·Î Á¦°øÇØ µå¸®°í ÀÖ½À´Ï´Ù. 100 test ºÐ·®À¸·Î 96-well plate ÇÑÆÇÀ» ÃæºÐÈ÷ ÇÏ°íµµ
³²´Â ºÐ·®ÀÔ´Ï´Ù. ±¸ÀÔ Àü¿¡ ¹Ì¸® ÃæºÐÈ÷ Å×½ºÆ® ÇØ º¸½Ã°í, ¸¸Á·ÇÏ½Ã¸é ±¸¸ÅÇØ ÁÖ¼¼¿ä.
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CCK-8 ¹«·á »ùÇà ½Åû ¹æ¹ý
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¾Æ·¡ÀÇ ¾ç½ÄÀ» ´Ù¿î·Îµå ¹ÞÀ¸½Å ÈÄ, ºóÄÀ» ä¿ö ¿õºñ ÇмúÆÀÀ¸·Î E-mailÀ» º¸³»Áֽðųª ÀüÈ Áֽøé
3~4ÀÏ ¾È¿¡ Åùè·Î ¹Þ¾Æº¸½Ç ¼ö ÀÖ½À´Ï´Ù (Àç°í ¼ÒÁø½Ã µû·Î ¿¬¶ô µå¸®°Ú½À´Ï´Ù).
»ùÇà ½Åû¼
´Ù¿î·Îµå
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°ü·ÃÀÚ·á ´Ù¿î·Îµå
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Protocol
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CCK-8 vs MTT
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Reference
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CCK-8ÀÇ ÀÛ¿ë ¿ø¸®¿Í
½ÇÇè°úÁ¤¿¡ ´ëÇØ ÀÚ¼¼È÷
¼³¸íÇÑ ÀÚ·áÀÔ´Ï´Ù.
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CCK-8°ú MTT µÎ ¹æ½ÄÀÇ
Â÷À̸¦ ¼³¸íÇÑ ÀÚ·áÀÔ´Ï´Ù.
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°¢ ¼¼Æ÷ÁÖ¿¡¼ CCK-8 È°¿ë¹ý
°ú Ãʱâ½ÇÇè¿¡ ÇÊ¿äÇÑ ¼¼Æ÷¼ö
¸¦ Á¤¸®ÇÑ ÀÚ·áÀÔ´Ï´Ù.
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°ü·ÃÁ¦Ç° ¾È³»
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Microbial Viability Assay Kit
Cat# M439-10
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- ¹ÚÅ׸®¾Æ¿ë CCK-8
- gram +/- , yeast ¿¡¼ »ç¿ë °¡´É
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Double Staining Kit (1 set)
Cat# CS01-10
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- live cell°ú dead cellÀ» Çü±¤À¸·Î double staining ÇÏ´Â kit
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LDH Assay Kit-WST
Cat# CK12-01 (100T)
Cat# CK12-05 (500T)
Cat# CK12-20 (2000T)
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- Dead cellÀ» ÃøÁ¤ÇÏ´Â CCK-8 kit
- ADCC, CDC assay Àû¿ë °¡´É
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HilyMax
Cat# H357-10 (1 ml)
Cat# H357-15 (5 ml)
Cat# H357-20 (10 ml)
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- Insect Cell¿¡ ÃÖÀûÈ µÈ transfection reagent
- ´Ù¾çÇÑ ¼¼Æ÷ÁÖ¿¡¼µµ ¿ì¼öÇÑ transfection efficiency.
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Tocriscreen (1120 compound)
Cat# 2884/1SET
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- ¼¼Æ÷ »ý¸® È°¼ºÀÌ °ËÁõµÈ 1120°¡Áö ÈÇÕ¹°À» ¸ð¾Æ³õÀº compound library
- Tocris¿¡¼¸¸ »ý»êµÇ´Â ÈÇÕ¹° »ó´ç¼ö Æ÷ÇÔ
- Cyto toxicity / proliferation screening¿¡ ÃÖÀûÀÇ ¾ÆÀÌÅÛ
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