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IC 제품군은 Intracellular Protein 정량이 가능한 제품으로 cell lysis에서 ELISA 방법으로 target protein을 detection합니다. 정량이 가능하므로 Western blot을 대체 할 수 있는 방법으로 주목 받고 있습니다.
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특징║Surveyor® IC ELISA kit
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제품 구성║Surveyor® IC ELISA kit
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- Pre-coated 96-well Microplate
- Conjugated Detection Antibody
- Calibrated Immunoassay Standard
- Cell Lysis Buffer
- Streptavidin-HRP
- Sample Diluent Concentrate
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- Reagent Diluent Concentrate
- Wash Buffer Concentrate
- Color Reagent A and B
- Stop Solution
- Plate Sealers
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특징║Duoset® IC ELISA Development System
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제품 구성║Duoset® IC ELISA Development System
- Capture Antibody
- Conjugated Detection Antibody
- Calibrated Immunoassay Standard
- Streptavidin-HRP
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추가 필요 품목║Duoset® IC ELISA Development System
- Aprotinin (Sigma #A6279)
- Leupeptin (Sigma # L8511)
- Pepstatin (Sigma # P4265)
- Phenylmethylsulfonylfluoride (PMSF) (Sigma # P7626)
- Sodium
azide (NaN3) (Sigma # S2002)
- Sodium
fluoride (NaF) (Sigma # 201154)
- Sodium
orthovanadate (Na3VO4) (Sigma # S6508), activated
- Sodium
pyrophosphate (Na4P2O7) (Sigma # P8010)
- Triton_ X-100
(Sigma # T9284)
- Urea
- 96
well microplates : Costar EIA Plates (Catalog # 2592)
or R&D Systems (Catalog # DY990)
- Platesealers (R&D Systems, Catalog # DY992)
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제품 원리║Surveyor®IC/ Duoset IC® ELISA
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A microplate pre-coated with capture antibody is provided. Samples or standards
are added and any analyte present is bound by the immobilized antibody. Unbound
materials are washed away.
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A second biotinylated antibody (detection antibody) is added and binds to the
captured analyte. Unbound detection antibody is washed away.
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Streptavidin-HRP is used to bind to the detection antibody. Unbound
streptavidin-HRP is washed away.
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Tetramethylbenzidine (TMB) substrate solution is added to the wells and a blue
color develops in proportion to the amount of analyte present in the sample.
Color development is stopped turning the color in the wells to yellow. The
absorbance of the color at 450 nm is measured.
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제품 종류║R&D
SYSTEMS ELISA kit
Complete ELISAs
ELISA Development Reagents
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내려 받기║R&D
SYSTEMS ELISA kit
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